ASSAY OF GRAYANOTOXINS BY GAS-CHROMATOGRA PHY

A gas chromatographic (GC) method was developed for the identification of grayanotoxins (G), I, II, and III, which are toxic diterpenoids of Leucothoe grayana MAX. GC was done with stainless steel capillary col umns (CB 17-M 15-025, 15 m, which corresponds to OV-17), with programm ing from 200 to 300-degrees-C at 10-degrees-C/min. Grayanotoxins with C3-OH and C6-OH gave split peaks on chromatograms. The concentration o f grayanotoxins and their GC peak area were directly proportional. Thi s method was used for the identification of grayanane compounds in tox ic honey.