IN-VITRO REGENERATION OF SHOOTS IN MATTHIOLA-INCANA L FROM SEEDLING EXPLANTS OF DIFFERENT AGE

In vitro regeneration of Matthiola incana L. was achieved from both yo ung explants from in vitro cultured seedlings (apical microcuttings an d leaves) and older tissues (leaves) from greenhouse grown plants. Onl y adventitious shoots formed, both indirectly from compact nodular cal lus, at the basal end of the microcuttings, and directly, from nodular structures on leaf explants. BA was critical to shoot regeneration an d the concentration required increased with the age of the tissue: 1 m g/l BA for young explants, 10 mg/l for older explants.