Simultaneous saccharification and fermentation (SSF) is recognized as
an efficient approach to the cost-effective conversion of biomass to f
uel ethanol. This methodology takes advantage of the relief in end-pro
duct inhibition realized by conducting cellulose hydrolysis and glucos
e fermentation in the same well-stirred vessel. In this study, 15 spec
ies of hardwoods and softwoods were collected from sawmills located in
the Appalachian region of the southern United States. These wood samp
les were air-dried to 8-10% moisture, pretreated using a dilute sulfur
ic acid cooking scheme at 160 degrees C, exhaustively washed, and appl
ied to SSF with Saccharomyces cerevisiae D,A. Although the glucan cont
ent of each wood was found to be relatively invariant throughout the s
amples tested, hemicellulosic sugar and lignin contents were unique to
each wood. These and other differences in chemical composition were r
elated to resulting ethanol yields from SSF.