Mw. Smith et al., GENOMIC SEQUENCE SAMPLING - A STRATEGY FOR HIGH-RESOLUTION SEQUENCE-BASED PHYSICAL MAPPING OF COMPLEX GENOMES, Nature genetics, 7(1), 1994, pp. 40-47
We present a simple and efficient method for constructing high resolut
ion physical maps of large regions of genomic DNA based upon sampled s
equencing. The physical map is constructed by ordering high density co
smid contigs and determining a sequence fragment from each end of ever
y clone. The resulting map, which contains 30-50% of the complete DNA
sequence, allows the identification of many genes and makes possible P
CR amplification of virtually any part of the genome. We apply this st
rategy to the automated analysis of the genome of the primitive eukary
ote Giardia lamblia and evaluate its applicability to the physical map
ping and DNA sequencing of the human genome.