GENOMIC SEQUENCE SAMPLING - A STRATEGY FOR HIGH-RESOLUTION SEQUENCE-BASED PHYSICAL MAPPING OF COMPLEX GENOMES

We present a simple and efficient method for constructing high resolut ion physical maps of large regions of genomic DNA based upon sampled s equencing. The physical map is constructed by ordering high density co smid contigs and determining a sequence fragment from each end of ever y clone. The resulting map, which contains 30-50% of the complete DNA sequence, allows the identification of many genes and makes possible P CR amplification of virtually any part of the genome. We apply this st rategy to the automated analysis of the genome of the primitive eukary ote Giardia lamblia and evaluate its applicability to the physical map ping and DNA sequencing of the human genome.