CHANGES OF SERUM AMYLASE, ITS ISOZYME FRACTIONS AND AMYLASE-CREATININE CLEARANCE RATIO IN DOGS WITH EXPERIMENTALLY-INDUCED ACUTE-PANCREATITIS

To investigate the diagnostic application of amylase to canine pancrea tic diseases, serum amylase activities, its isozyme fractions and amyl ase-creatinine clearance ratio (ACCR) were analyzed in normal intact d ogs and dogs experimentally induced acute pancreatitis. There was no s tatistic difference between normal male and female dogs. Amylase speci fic activities in pancreatic tissue extracts were more than 2,300 time s higher than that in serum, and were also higher than those in other tissues; parotid and mandibular salivary glands, lung, heart, liver, s pleen, duodenum, jejunum, ileum and kidney. Following the chloroform i njection into the pancreatic tissue, WBC increased from 6 to 240 hr an d serum glucose significantly increased at 72 and 96 hr, and no urine glucose was detected. BUN as well as serum and urine creatinine showed normal levels. ACCR increased until 96 hr without statistic significa nce. Serum amylase activities increased significantly after 3 hr and i ts isozyme was separated into 4 fractions (Amy1-Amy4) in contrast to 3 fractions (Amy2-Amy4) in intact dogs. Since this extra Amyl seen from 1 hr increasing after 6 hr similarly to other 3 fractions, the evalua tion of serum amylase and its isozyme fractions was indicated to be us eful for the diagnosis of acute pancreatitis in dogs.