SIMPLE AND EFFICIENT PURIFICATION OF C-REACTIVE PROTEIN FROM CANINE SERUM

Simple purification method for canine serum, C-reactive protein (CRP) was established. By combining an affinity chromatography on Sepharose 4B coupled with o-phosphorylethanolamine and a gel filtration on a col umn of Sephacryl S-300, about 9 mg of purified CRP from 50 ml of canin e serum was obtained. The method is simple and efficient to purify can ine CRP.