HUMAN VASCULAR ENDOTHELIAL-CELLS ON EXPANDED PTFE PRECOATED WITH AN ENGINEERED PROTEIN ADHESION FACTOR

Citation
Jp. Mazzucotelli et al., HUMAN VASCULAR ENDOTHELIAL-CELLS ON EXPANDED PTFE PRECOATED WITH AN ENGINEERED PROTEIN ADHESION FACTOR, International journal of artificial organs, 17(2), 1994, pp. 112-117
Citations number
25
Categorie Soggetti
Engineering, Biomedical
ISSN journal
03913988
Volume
17
Issue
2
Year of publication
1994
Pages
112 - 117
Database
ISI
SICI code
0391-3988(1994)17:2<112:HVEOEP>2.0.ZU;2-C
Abstract
To elucidate the role of the molecular structure of adhesive proteins in an endothelialization of synthetic vascular prosthesis in vitro, a recombinant fibronectin-like engineered adhesion factor (FP) construct ed from the specific Arg-Gly-Asp cell adhesion repeats was assayed as adhesive substrate to culture human saphenous vein endothelial cells o n ePTFE. ePTFE samples (I cm(2)) inserted into cell culture chambers w ere coated by incubation with increasing amounts of FP (up to 100 mu g /cm(2)) prior to cell seeding. At 24 hours after low density cell seed ing and 20 mu g/ml/cm(2) FP concentration, the number of adhered cells reached a plateau and the adhered cells did not proliferate up to 6 d ays of culture. At 24 hours after high density seeding (10(5) cells/cm (2)), the number of adhered cells was significantly higher on ePTFE wi th preadsorbed FP than on fibronectin coated PTFE. About 55% of the in itially adhered cells survived up to 7 days on FP, whereas cell debris and free nuclei were predominant on fibronectin coated PTFE. In the i nvestigated model the engineered RGD polymer potentialized a short-ter m adhesion of vascular endothelial cells to PTFE, nevertheless it did not ensure proliferation and long-term survival of these cells.