IDENTIFICATION OF LECTIN ACTIVITY IN THE HEMOLYMPH OF CASTNIA-LICUS DRURY, A SUGARCANE GIANT BORER (LEPIDOPTERA-CASTNIIDAE)

Authors
ANTUNES RV COELHO LCBB
Citation
Rv. Antunes et Lcbb. Coelho, IDENTIFICATION OF LECTIN ACTIVITY IN THE HEMOLYMPH OF CASTNIA-LICUS DRURY, A SUGARCANE GIANT BORER (LEPIDOPTERA-CASTNIIDAE), Applied biochemistry and biotechnology, 47(1), 1994, pp. 33-37
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
47
Issue
1
Year of publication
1994
Pages
33 - 37
Database
ISI
SICI code
0273-2289(1994)47:1<33:IOLAIT>2.0.ZU;2-Z
Abstract
Castnia licus Drury, in the larvae stage, is a major pest in the sugar cane industry of Northeastern Brazil (giant borer). A natural hemagglu tination activity was detected in the larvae hemolymph of this insect and the activity have not been inhibited by tested carbohydrates. Frac tionation with ammonium sulfate showed that the supernatant of the 15- 30% fraction (S15-30%) had the lectin's highest specific activity. Aci dic and basic lectin activities were separated by chromatography on CM -cellulose; the fractionated molecular forms showed distinct electroph oresis mobilities.