K. Zheng et al., RFLP BASED PHYLOGENETIC ANALYSIS OF WIDE COMPATIBILITY VARIETIES IN ORYZA-SATIVA L, Theoretical and Applied Genetics, 88(1), 1994, pp. 65-69
Twenty-one wide compatibility varieties (WCVs) of rice together with t
hree indica and three japonica testers were assayed with 160 DNA probe
s that were selected to represent the entire RFLP map at an average in
terval of 11 cM. On the basis of four enzyme digestion 125 probes dete
cted polymorphisms among the WCVs and subspecies' testers. Among these
polymorphic probes there were 68 that could distinguish the indica fr
om the japonica testers. Two dendrograms were constructed on the basis
of 398 polymorphic fragments of 125 probes and 139 polymorphic fragme
nts of 68 subspecies' differentiating probes in combination with singl
e enzymes, respectively. The reliability and representativeness of the
testers and the levels of DNA variations among WCVs were estimated. T
he potential of WCVs in the utilization of intersubspecific heterosis
is discussed.