M. Simoni et al., SCREENING FOR DELETIONS OF THE Y-CHROMOSOME INVOLVING THE DAZ (DELETED IN AZOOSPERMIA) GENE IN AZOOSPERMIA AND SEVERE OLIGOZOOSPERMIA, Fertility and sterility, 67(3), 1997, pp. 542-547
Objective: To evaluate the occurrence and prevalence of microdeletions
of the Y chromosome involving the DAZ (Deleted in AZoospermia) gene i
n patients with azoospermia or severe oligozoospermia. Design: Control
led clinical study. Setting: University infertility clinic. Patient(s)
: Infertile men (n = 168) with nonobstructive, idiopathic azoospermia
or severe oligozoospermia and normal LH. The control group consisted o
f proven fathers (n = 86). Intervention(s): None. Main Outcome Measure
(s): Semen analysis; polymerase chain reaction amplification of the lo
ci sY84, sY143, sY254, and sY255; serum FSH, LH, and T; testicular vol
ume. Result(s): Deletions involving the sY254 and sY255 DAZ loci were
found in three azoospermic patients and two men with sperm concentrati
on <1 x 10(6)/mL. Serum FSH was elevated in four patients and was norm
al in one. All five patients had decreased testicular volumes compared
with controls. No deletions involving the sY84 and sY143 loci were fo
und. The four loci were amplified normally in the control group. Concl
usion(s): The estimated frequency of deletions involving the DAZ locus
is 3% in azoospermic-severely oligozoospermic men consulting an infer
tility clinic. Polymerase chain reaction amplification of the DAZ locu
s is useful for the diagnosis of microdeletions of the Y chromosome. D
eletions involving more proximal regions of the Y chromosome seem to b
e rare.