ELECTROTRANSFER (WESTERN-BLOT) OF FOOT-AND-MOUTH-DISEASE VIRUS C3-RESENDE SUBTYPE CAPSID PROTEINS AND THEIR REACTIVITY WITH MONOCLONAL-ANTIBODIES

Capsid proteins (VP1, VP2, VP3 and VP4) of the foot-and-month disease virus C3 Resende subtype were separated by gel electrophoresis and wer e electro transferred (Western blot) and immobilized on the membrane. A set of 26 monoclonal antibodies was used for examination of the reac tivity with the above mentioned capsid proteins, whereafter reactivity with 12 monoclonal antibodies with VP1 was recorded. No reactivity wa s observed with any other viral capsid protein. Differences between se quentional and conformational epitopes on the surface of the virus ant igen are discussed.