MULTIPLE FRAGMENT LIGATION ON GLASS-SURFACE - A NOVEL-APPROACH

Binding of DNA to pure silicon dioxide involving formation of hydrogen bonds by disruption of the hydration shell of DNA using chaotropic ag ents can be easily reversed with water or buffers of low ionic strengt h. When powdered glass was used instead of pure silicon dioxide the bi nding of DNA to glass was less easily reversed. DNA bound to glass per mits digestion by restriction enzyme, ligation and transformation. The method opens up the possibility of enhancing transformation efficienc y especially for DNA digests containing more than two pieces.