MEASUREMENT OF THE RATE OF UPTAKE AND SUBCELLULAR-LOCALIZATION OF PORPHYRINS IN CELLS USING FLUORESCENCE DIGITAL IMAGING MICROSCOPY

Authors
GEORGIOU GN AHMET MT HOULTON A SILVER J CHERRY RJ
Citation
Gn. Georgiou et al., MEASUREMENT OF THE RATE OF UPTAKE AND SUBCELLULAR-LOCALIZATION OF PORPHYRINS IN CELLS USING FLUORESCENCE DIGITAL IMAGING MICROSCOPY, Photochemistry and photobiology, 59(4), 1994, pp. 419-422
Citations number
11
Categorie Soggetti
Biophysics,Biology
Journal title
Photochemistry and photobiology → ACNP
ISSN journal
00318655
Volume
59
Issue
4
Year of publication
1994
Pages
419 - 422
Database
ISI
SICI code
0031-8655(1994)59:4<419:MOTROU>2.0.ZU;2-5
Abstract
A fluorescence imaging system incorporating a cooled slow-scan charge- coupled device camera was used to study the rate of uptake and subcell ular localization of porphyrins in living cells. Measurements were car ried out on human dermal fibroblasts (D532) using two different porphy rins meso-tetra(4-N-methylpyridyl)porphine (TMPP) and meso-tetra(4-N-h exylpyridyl)porphine (THPP). It was observed that TMPP was rapidly tak en up by cells and principally located in the nucleus. The THPP, on th e other hand, internalized more slowly and exhibited a particulate dis tribution in the cytoplasm.