PHOTOLYSIS OF INTACT YOUNG HUMAN, BABOON AND RHESUS-MONKEY LENSES

Intact young human, baboon and rhesus monkey lenses were subjected to near-UV irradiation under identical conditions and fluorophore buildup was continuously monitored for several hours. The compositional chang es occurring in the lenses were monitored by analyzing the ethanol ext racts of the irradiated and control lenses using high-performance liqu id chromatography and thin-layer chromatography (TLC). The chromatogra ms of the supernatant detected at 365 nm as well as the TLC scans show ed the presence of 3-hydroxykynurenine glucoside (3-HKG) and two other kynurenine-type compounds. The 3-HKG and one of the compounds were fo und in all three species, while the remaining one was structurally dif ferent in the lower primates. A loss of 3-HKG as a result of irradiati on was apparent in all the lenses and correlated with the buildup of t he blue fluorophore, suggesting that the latter may be a photoproduct( s) of 3-HKG. The kinetic analysis of baboon and human lenses showed a slowdown in the fluorophore buildup as irradiation times increased. Th is was probably due to the competitive absorptions of 3-HKG and other chromophores present. Rhesus monkey lenses did not exhibit this slowdo wn.