A COMBINED STUDY OF HUMAN-COMPLEMENT C7 IEF AND C7 M N POLYMORPHISMS IN THE CHINESE HAN POPULATION/

Two polymorphisms of human complement C7 (IEF and M/N) have been studi ed in the Han population from Chengdu, People's Republic of China, One was determined by polyacrylamide gel isoelectric focusing (IEF) of ne uraminidase-treated EDTA plasma, whereas the other was performed by a combination of two ELISAs using a C7-allospecific monoclonal antibody or polyclonal C7-specific IgG as coating antibodies. In 121 Chinese su bjects IEF patterns of C7 were classified into three common and four r are phenotypes. The C7 IEF allele frequency estimates for C71, C7*2, C73, C7*4 and C7*7 were 0.831, 0.095, 0.045, 0.025 and 0.004, respect ively. The C7 M/N typing in 113 Chinese individuals revealed the follo wing allele frequencies: C7M = 0.805, C7*N = 0.195. Allelic associati ons between C7 IEF alleles and the C7 M/N alleles are also discussed.