La. Brauns et Jd. Oliver, POLYMERASE CHAIN-REACTION OF WHOLE-CELL LYSATES FOR THE DETECTION OF VIBRIO-VULNIFICUS, Food biotechnology, 8(1), 1994, pp. 1-6
A quantitative, efficient, and rapid method of detecting cells of the
human pathogen, Vibrio vulnificus, using the polymerase chain reaction
is described. The technique employs whole cell lysates as the source
of template DNA, and amplifies the cytotoxin/hemolysin gene. Using pri
mers internal to this gene, we were able to detect a single cell of V.
vulnificus. This method, which does not require DNA extraction or use
of a probe, may facilitate the detection of low levels of this pathog
en when present in shellfish.