PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST EPITOPES OF THE MAIN COATPROTEIN OF FILAMENTOUS FD PHAGES

Authors
MICHEEL B HEYMANN S SCHARTE G BOTTGER V VOGEL F DUBEL S BREITLING F LITTLE M BEHRSING O
Citation
B. Micheel et al., PRODUCTION OF MONOCLONAL-ANTIBODIES AGAINST EPITOPES OF THE MAIN COATPROTEIN OF FILAMENTOUS FD PHAGES, Journal of immunological methods, 171(1), 1994, pp. 103-109
Citations number
22
Categorie Soggetti
Immunology
Journal title
Journal of immunological methods → ACNP
ISSN journal
00221759
Volume
171
Issue
1
Year of publication
1994
Pages
103 - 109
Database
ISI
SICI code
0022-1759(1994)171:1<103:POMAEO>2.0.ZU;2-W
Abstract
Three monoclonal antibodies (MAbs) were produced which react with epit opes of the main structural coat protein (pVIII) of filamentous fd pha ges as demonstrated by solid-phase fluorometric enzyme immunoassays an d by immunoelectron microscopy. The antibodies are of the IgG1, IgG2a and IgG2b immunoglobulin subclasses. Since they also react with recomb inant phages expressing antigen fragments in their pIII region they ma y be suitable reagents for the demonstration and isolation of filament ous phages used in recombinant protein technology.