F. Gonzalez et al., LOCALIZATION OF ACID AND ALKALINE-PHOSPHATASES IN MYXOCOCCUS CORALLOIDES-D, Letters in applied microbiology, 18(5), 1994, pp. 264-267
Myxococcus coralloides produces two different phosphatases, one acid a
nd the other alkaline. Both enzymes were localized by physical and bio
chemical techniques. Spheroplasts from M. coralloides released 20-30%
of the phosphatase activities. Osmotic shock or treatment with high Mg
Cl2 or LiCl concentrations did not produce a greater release. Cytochem
ical localization situated the phosphatases in the outer membrane and
the periplasmic space. Separation of the cytoplasmic membrane and oute
r membrane of the cells by sucrose gradient centrifugation showed that
phosphatases are located primarily in the outer membrane. membrane.