The aim of this study was to produce viable chicks by in vitro fertili
zation and transfer of fertilized ova to the oviduct of recipient hens
. Out of a total of 76 transferred ova, 53 were laid with fully calcif
ied shells, 31 of which were fertile (58%). Despite the high rate of e
mbryonic loss, six live chicks were hatched from 12 fertile ova expose
d to 0.05 mi of semen (1:200 dilution). Nine healthy chicks were hatch
ed from ten control ova which were recovered from the oviduct followin
g artificial insemination and subsequent transfer to recipient hens. T
his experimental approach provides a useful model for production of tr
ansgenic chicks.