FREE-RADICAL FORMATION IN THE INTERACTION OF HYPOCHLORITE WITH IRON(II) IONS - A CHEMILUMINESCENT STUDY

Hypochlorite interaction with ferrous ions was investigated by means o f chemiluminescence assay. It was demonstrated that HOCl and Fe2+ reac tion produces a fast flash with the maximal amplitude at 1 second. The comparison of the chemiluminescent spectra obtained in reactions of F e2+ with HOCl and H2O2 demonstrated that both spectra bad their maxima l absorbance in the region 500 - 510 nm. The addition of a spin trap ( C-phenyl-N-tert-butyl nitrone) quenched chemiluminescence in both syst ems in a concentration dependent manner. The obtained results allowed us to suggest that the nature of the particles produced in the reactio ns of Fe2+ with HOCl or H2O2 is simular.