A STREPTAVIDIN-CELLULOSE-BINDING DOMAIN FUSION PROTEIN THAT BINDS BIOTINYLATED PROTEINS TO CELLULOSE

A fusion protein, Sta-CBDCex, which comprises streptavidin with a cell ulose-binding domain (CBDCex) fused to its C terminus, was produced in the cytoplasm of Escherichia coli where it formed inclusion bodies. R enatured Sta-CBDCex, recovered from the inclusion bodies, adsorbed to Avicel, a microcrystalline cellulose. The cellulose bound Sta-CBDCex i n turn bound biotinylated alkaline phosphatase or biotinylated P-gluco sidase. The immobilized beta-glucosidase remained fully active during 2 weeks of continuous column operation at 50 degrees C.