ANTI-DNA-[PT(DIEN)CL]CL ANTIBODIES FOR DE TECTION OF SPECIFIC DNA-SEQUENCES

Authors
KISELEVA VI TURCHINSKY MF KOLESNIK TB POVERENNY AM
Citation
Vi. Kiseleva et al., ANTI-DNA-[PT(DIEN)CL]CL ANTIBODIES FOR DE TECTION OF SPECIFIC DNA-SEQUENCES, Bioorganiceskaa himia, 20(1), 1994, pp. 14-20
Citations number
12
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Bioorganiceskaa himia → ACNP
ISSN journal
01323423
Volume
20
Issue
1
Year of publication
1994
Pages
14 - 20
Database
ISI
SICI code
0132-3423(1994)20:1<14:AAFDTO>2.0.ZU;2-Y
Abstract
Monofunctional platinum compound [Pt(dien)Cl]Cl and high-affinity anti bodies against DNA-[Pt(dien)Cl]Cl were suggested for non-radioactive h ybridisation analysis of DNA. The simple labelling procedure was based on mixing the reagents followed by the incubation for 2 h at 60 degre es C. The sensitivity and specificity of the technique were sufficient to detect 10 fg DNA in dot-hybridisation without cross-reaction with 10-fold exess of a heterological DNA. This technique permits genome li braries screening,