EFFECT OF TORDON 2,4-D 64 240 TRIETHANOLAMINE BR ON THE ENERGY-METABOLISM OF RAT RENAL CORTICAL MITOCHONDRIA/

Tordon herbicide (4-amino-3,5,6-trichloropicolinic acid -picloram, and 2,4-dichlorophenoxyacetic acid - 2,4-D) depresses the phosphorylation efficiency of rat renal cortical mitochondria as deduced from the dec rease of respiratory control (RCR) and ADP/0 ratios when NAD+-dependen t substrates were used; NADH oxidase (50%) and NADH cytochrome c reduc tase (23%) were also inhibited. Tordon had no effect on the other enzy matic complexes of the respiratory chain. The amplitude and the veloci ty of swelling induced by glutamate is progressively inhibited, while with succinate, TMPD (N,N,N1,N1-tetramethyl-p-phenylediamine) + sodium ascorbate and ATP as substrate a partial inhibition of the amplitude was observed. Tordon promote a decrease in the membrane potential upon energization of the mitochondrial membrane only by glutamate. These r esults characterize an interaction of Tordon with complex 1 of proton- motive force of the mitochondrial inner membrane without affecting its elasticity.