SEPARATION OF ENANTIOMERS AND DIASTEREOMERS OF 4-HYDROXY-2H-1,4-BENZOXAZIN-3(4H)-ONE DERIVATIVES BY CAPILLARY ELECTROPHORESIS

A high performance capillary electrophoresis (HPCE) procedure for the enantioseparation of the methyl acetals 3 and 4 of the natural product s DIBOA 1 and DIMBOA 2, respectively, has been developed using berate buffers (pH 9-10 range), cyclodextrins as chiral additives and additio n of up to 20 % methanol. A mixture of GDIBOA 5 and GDIMBOA 6 of natur al origin was also clearly separated. HPCE proved to be superior to HP LC by the first separation of GDIBOA 5 and three of its diastereomers resulting from a synthetic approach to this acetalglucoside.