INFLUENZA-VIRUS M2 PROTEIN MODIFIES MEMBRANE-PERMEABILITY IN ESCHERICHIA-COLI-CELLS

Authors
GUINEA R CARRASCO L
Citation
R. Guinea et L. Carrasco, INFLUENZA-VIRUS M2 PROTEIN MODIFIES MEMBRANE-PERMEABILITY IN ESCHERICHIA-COLI-CELLS, FEBS letters, 343(3), 1994, pp. 242-246
Citations number
33
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
343
Issue
3
Year of publication
1994
Pages
242 - 246
Database
ISI
SICI code
0014-5793(1994)343:3<242:IMPMMI>2.0.ZU;2-I
Abstract
The M2 protein of influenza virus is an integral membrane protein with ion channel activity. This protein has been expressed in E. coil cell s in an inducible manner. Expression of the M2 protein causes rapid ly sis of BL21(DE3) pLysS E. coil cells upon induction with IPTG. M2 prot ein increases membrane permeability to a number of hydrophylic molecul es, such as ONPG, uridine or impermeant translation inhibitors. The be haviour of M2 in bacteria resembles that of other viral proteins, such as poliovirus 3A and Semliki Forest virus 6K.