ENHANCED STABILITY OF AN L-HYDANTOINASE MEDIATED BY ITS CORRESPONDINGPOLYCLONAL ANTIBODY

Authors
SIEMANN M SYLDATK C WAGNER F
Citation
M. Siemann et al., ENHANCED STABILITY OF AN L-HYDANTOINASE MEDIATED BY ITS CORRESPONDINGPOLYCLONAL ANTIBODY, Biotechnology letters, 16(4), 1994, pp. 349-354
Citations number
15
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
16
Issue
4
Year of publication
1994
Pages
349 - 354
Database
ISI
SICI code
0141-5492(1994)16:4<349:ESOALM>2.0.ZU;2-F
Abstract
Polyclonal antibodies were produced against the highly purified L-5-ar yl-alkyl-hydantoinase and hydantoin-racemase of Arthrobacter aurescens DSM 3747. Serological interaction of the hydantoinase antibody with i ts corresponding antigenic fraction let to a remarkable improvement of the stability of the hydantoinase. The immobilization of the catalyti c active enzyme-antibody complex onto a Sepharose 4B-support underline d this antibody-mediated stabilization effect, while control experimen ts with preimmunsera or the hydantoin-racemase antibodies gave no or e ven a negative effect.