Lipase biosynthesis occured in medium without lipids, but for improved
production an inducer was needed. The source and concentration of an
inducer had no significant effect. Starch as an additional carbon sour
ce stimulated lipase biosynthesis when used in small amounts. Addition
of NH4NO3 as a nitrogen source, KH2PO4 as a phosphate source as well
as Mg ions to the medium with inital pH 5.0 gave the best yield.