IN-VIVO FLUORESCENCE MICROSCOPY OF MICROCIRCULATION IN THE RENAL-CORTEX OF MICE .5. EFFECTS OF MANNITOL AND INFUSIONS IN NORMAL, OBESE HYPERGLYCEMIC AND DIABETIC MICE/

In vivo fluorescence microscopy was used in experimental studies of re nal cortical microcirculation in mice. The effects of i.v. infusions o f mannitol and iohexol were studied in normal and obese/hyperglycemic mice and in mice with streptozotocin-induced diabetes mellitus. All in fusions produced marked effects on the distribution and velocity of co rtical blood flow with no differences between iohexol and mannitol. Th e renal cortical blood flow was inhomogeneous regarding different capi llaries and there was an initial rise in the number of capillaries wit h increased blood flow after infusions of both mannitol and iohexol. T he infusions of contrast medium were initially followed by a significa ntly larger number of capillaries with decreased blood flow in the obe se/hyperglycemic mice (p < 0.05) than in the diabetic and nondiabetic mice. The variations in obese/hyperglycemic groups were, however, larg e. The effects were the same in normal and diabetic animals.