OPTIMIZED BIOSENSOR FOR WHOLE-BLOOD MEASUREMENTS USING A NEW CELLULOSE-BASED MEMBRANE

We used bacterial cellulose membranes as outer membranes for glucose s ensors based upon amperometric detection of glucose oxidase reaction p roducts. We found a long-term stability in 1:10 diluted blood of about 200 h. In comparison a Cuprophan(R) membrane was stable for only 30 h under the same conditions. Comparing the two membranes in undiluted h uman blood, a similar trend was found in the long-term stability: Cupr ophan(R) was stable for only 3-4 h and the glucose sensor with the bac terial cellulose membrane was stable for more than 24 h. In addition, the measuring range of the glucose sensor could be extended up to 170 mM glucose by treating the bacterial cellulose membrane with a polyami de.