RECOGNITION OF UUN CODONS BY 2 LEUCINE TRANSFER-RNA SPECIES FROM ESCHERICHIA-COLI

Authors
TAKAI K HORIE N YAMAIZUMI Z NISHIMURA S MIYAZAWA T YOKOYAMA S
Citation
K. Takai et al., RECOGNITION OF UUN CODONS BY 2 LEUCINE TRANSFER-RNA SPECIES FROM ESCHERICHIA-COLI, FEBS letters, 344(1), 1994, pp. 31-34
Citations number
30
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
344
Issue
1
Year of publication
1994
Pages
31 - 34
Database
ISI
SICI code
0014-5793(1994)344:1<31:ROUCB2>2.0.ZU;2-N
Abstract
Codon recognition by Escherichia coli tRNA(4)(Leu) and tRNA(5)(Leu) wa s investigated by analysis of the competition between two aminoacyl-tR NA species in an in vitro protein synthesis. Both tRNA species strictl y obey the wobble rule when they are in competition with other tRNA sp ecies. This is probably due to the post-transcriptional modifications at the first position of the anticodon of these tRNA(Leu) species, sup porting the proposal that the conformational rigidity of post-transcri ptionally modified pyrimidine nucleotides guarantees the correct codon recognition.