The rate of P-32(i) incorporation into the main membrane phospholipid
fractions, i.e. phosphatidylcholine (PC), phosphatidylethanolamine (PE
) and phosphatidylinositol (PI), as well as their contents in the cell
s during synchronous growth of the myxomycete, Physarum polycephalum,
have been studied. It has been found that both the phospholipid levels
and the rates of P-32(i) incorporation increase during the S phase ti
ll the early G(2) phase, remain nearly constant during the G(2) phase
and fall to the initial level at the end of the G(2) phase and in mito
sis. It has been revealed that the rate of P-32(i) incorporation into
PC is very low compared to PE and PI, in spite of the fact that the PC
level is the highest. The possible reasons of this phenomenon are dis
cussed.