Bb. Trifonov et al., BIOLOGICAL EFFECTS OF A NOVEL INTESTINAL PEPTIDE - INHIBITING ENTEROCYTOGENIN ON CULTURED 3T3 MOUSE FIBROBLASTS AND L5178Y MOUSE LYMPHOMA-CELLS, Regulatory peptides, 51(2), 1994, pp. 111-119
The effects of a new intestinal peptide, inhibiting enterocytogenin (I
EG) derived from pig intestinal mucosa were studied in vitro on 3T3 mo
use fibroblasts and L5178Y mouse lymphoma cell line. IEG caused consid
erable growth inhibition together with specific morphological changes,
necrotic effects as well as formation of monolayers at the highest co
ncentration applied (1000 mu g/ml). A biologically active fraction (IE
G-BAF) derived by further purification of IEG by gel-filtration, prove
d to possess most of the described activity. The concentrations of IEG
and IEG-BAF inhibiting the growth of L5178Y lymphoma cells by 50%, (I
C50 values) were calculated to be 759 mu g/ml and 19? mu g/ml, respect
ively. IEG-BAF has a molecular mass of 4450 +/- 180 Da and is most pro
bably a peptidylnucleotidate as revealed by spectral analysis.