2 MOLECULAR-FORMS OF PEPTIDE YY (PYY) ARE ABUNDANT IN HUMAN BLOOD - CHARACTERIZATION OF A RADIOIMMUNOASSAY RECOGNIZING PYY-1-36 AND PYY-3-36

Authors
GRANDT D SCHIMICZEK M BEGLINGER C LAYER P GOEBELL H EYSSELEIN VE REEVE JR
Citation
D. Grandt et al., 2 MOLECULAR-FORMS OF PEPTIDE YY (PYY) ARE ABUNDANT IN HUMAN BLOOD - CHARACTERIZATION OF A RADIOIMMUNOASSAY RECOGNIZING PYY-1-36 AND PYY-3-36, Regulatory peptides, 51(2), 1994, pp. 151-159
Citations number
39
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Regulatory peptides → ACNP
ISSN journal
01670115
Volume
51
Issue
2
Year of publication
1994
Pages
151 - 159
Database
ISI
SICI code
0167-0115(1994)51:2<151:2MOPY(>2.0.ZU;2-3
Abstract
Two endogenous forms of PW are abundant in man and dog, PW 1-36 (PYY-I ) and PYY 3-36 (PYY-II). PW-II is a major molecular form of PW in huma n colon, but it is not known, whether PYY-II is also released into the circulating blood. Several radioimmunoassays for measuring PYY-I in p lasma have been developed, but it has not been reported, whether they equally detect PYY-II, We characterize a radioimmunoassay for measurin g PYY in human plasma which equally recognizes PW-I and PYY-II. Using this radioimmunoassay and reversed phase HPLC we demonstrate the exist ence of two forms of PYY in human blood, coeluting with synthetic PW-I and PW-II.