M. Teige et al., PROBLEMS WITH SERUM-FREE PRODUCTION OF ANTITHROMBIN-III REGARDING PROTEOLYTIC ACTIVITY AND PRODUCT QUALITY, Journal of biotechnology, 34(1), 1994, pp. 101-105
Human antithrombin III (AT-III) was produced using a recombinant BHK-2
1 cell line with a microcarrier culture in spinner flasks. Cells were
cultivated for the first 4 days in a medium containing 10% fetal calf
serum (FCS). Afterwards, the medium was exchanged and production of AT
-III occurred at high cell numbers in a serum-free medium. The product
was determined by an immunoassay and further analysed after isolation
from the culture medium. During cultivation, high proteolytic activit
y was detected which caused a considerable product decomposition. Furt
hermore, a higher level of non-glycosylated AT-III was found after ser
um-free production.