DIFFERENTIAL ADENOASSOCIATED VIRUS VECTOR-DRIVEN EXPRESSION OF A NEUROPEPTIDE-Y GENE IN PRIMARY RAT-BRAIN ASTROGLIAL CULTURES AFTER TRANSFECTION WITH SENDAI VIROSOMES VERSUS LIPOFECTIN(TM)

The ability of Sendai virosomes or Lipofectin(TM) to introduce an AAV vector into primary rat brain astroglial cultures was characterized. T he pJDT95npy vector was constructed by inserting rat NPY cDNA downstre am from the indigenous AAV p5, p19 and p40 promoters in pJDT95. Lipofe ctin(TM)-mediated transfection with pJDT95npy (10 mu g) resulted in pr onounced expression of several NPY mRNA species: p5-driven (3.3 kb), p 19-driven (2.7 kb) and p40-driven (0.6, 0.8, 1.1, and 1.8 kb). Exposur e to virosomally encapsulated pJDT95npy (50 or 100 ng) resulted in tra nsient expression of some p40-driven mRNA species (0.8 and 1.8 kb). Ne ither method produced astroglia cells which synthesized mature NPY imm unoreactivity. This demonstrates that an AAV-derived vector can drive gene expression in astroglia, that Sendai virosomes can infuse vectors into astroglia, but that the amount of DNA infused in this manner may limit long term expression.