OXOLINIC ACID AND FLUMEQUINE IN FISH-TISSUES - VALIDATION OF AN HPLC METHOD - ANALYSIS OF MEDICATED FISH AND COMMERCIAL FISH SAMPLES

This paper describes a simple method for residue analysis of oxolinic acid and flumequine in fish tissues by HPLC and fluorometric detection . The quinolones were extracted with ethyl acetate, purified with hexa ne and then analyzed using a RP-8 Lichrosorb reversed phase column. Th e method was validated with satisfying results. The high sensitivity o f the fluorometric detection allowed to reach a quantification limit o f 2 mu g oxolinic acid and 5 mu g flumequine/kg fish tissue and an abs olute detection limit of 20 pg and 50 pg respectively. Medicated fish as well as commercial cultured fish samples were analyzed with the des cribed method. Oxolinic acid and flumequine were detected in both of t hem.