SEPARATION OF ACROLEIN AND ITS POSSIBLE METABOLITES USING DIFFERENT MODES OF HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Ion-exclusion, reverse phase, anion exchange and partition chromatogra phy were used to separate acrolein and related compounds including 3-h ydroxypropanal, 3-hydroxypropionic acid, 1,3-propanediol, allyl alcoho l, acrylic acid, HCO3, glycerol, glycidol, oxalic acid, malonic acid, propionic acid, ethanol, and propanol. The ion-exclusion chromatograph y employed an Interaction ORH-801 organic acid analysis column and was capable of separating these small and polar molecules. The reverse ph ase chromatography was used to resolve co-eluting compounds on the ion -exclusion column. The anion exchange and partition chromatography wer e used to separate organic acids and carbohydrates, respectively.