LIPASE PRODUCTION FROM HYDROCARBONS BY TRICHOSPORON FERMENTANS WU-C-12 IN THE PRESENCE OF SURFACTANTS

When Trichosporon fermentans WU-C12 was cultivated for 3-4 days in med ia containing 3% (v/v) kerosene and 0.8% (v/v) Tween 85, 3% (v/v) gas oil and 0.6% (v/v) Triton X-405, and 3% (v/v) liquid paraffin and 0.6% (v/v) Span 80, the extracellular lipase activities reached 58-62 U/ml , approximately 2-3 times as much as that without any addition of surf actant. The extracellular and total (intra- plus extracellular) lipase activities were affected by the combination of surfactants and petrol eum products as the carbon sources.