THE ROLE OF CYTOCHROME-P450 2C3 IN RABBIT LIVER MICROSOMAL METABOLISMOF 1-NITROPYRENE AND 3-NITROFLUORANTHENE

Authors
HOWARD PC MCMANUS ME KOOP DR
Citation
Pc. Howard et al., THE ROLE OF CYTOCHROME-P450 2C3 IN RABBIT LIVER MICROSOMAL METABOLISMOF 1-NITROPYRENE AND 3-NITROFLUORANTHENE, Journal of biochemical toxicology, 9(2), 1994, pp. 71-78
Citations number
34
Categorie Soggetti
Biology
Journal title
Journal of biochemical toxicology → ACNP
ISSN journal
08872082
Volume
9
Issue
2
Year of publication
1994
Pages
71 - 78
Database
ISI
SICI code
0887-2082(1994)9:2<71:TROC2I>2.0.ZU;2-G
Abstract
The male rabbit liver microsomal cytochrome P450 metabolism of 1-nitro pyrene and 3-nitrofluoranthene was investigated. In this study, we use d inhibitory antibodies specific for rabbit P450 2C3 and determined th at, in untreated male rabbit liver microsomes, the antibody inhibited approximately 75% of the cytochrome P450-mediated C-oxidation of both 1-nitropyrene and 3-nitrofluoranthene. These results verify our previo us prediction that mainly one cytochrome P450 is responsible for micro somal metabolism of 1-nitropyrene in the untreated rabbit liver.