ALPHA-GLUCURONIDASE-PRODUCING ABILITY OF BASIDIOMYCETES USING 2-O-(ALPHA-D-GLUCOPYRANOSYLURONIC ACID)-D-XYLOSE AND P-NITROPHENYL ALPHA-D-GLUCOPYRANOSYLURONIC ACID AS SUBSTRATES
Citation
Y. Kawabata et al., ALPHA-GLUCURONIDASE-PRODUCING ABILITY OF BASIDIOMYCETES USING 2-O-(ALPHA-D-GLUCOPYRANOSYLURONIC ACID)-D-XYLOSE AND P-NITROPHENYL ALPHA-D-GLUCOPYRANOSYLURONIC ACID AS SUBSTRATES, Mokuzai Gakkaishi, 40(3), 1994, pp. 336-339
Categorie Soggetti
Materials Science, Paper & Wood
SICI code
0021-4795(1994)40:3<336:AAOBU2>2.0.ZU;2-G
Abstract
This work was performed to test the alpha-glucuronidase-producing abil
ity of basidiomycetes (70 species) using 2-O-(alpha-D-glucopyranosylur
onic acid)-D-xylose (GA-2X) and p-nitrophenyl alpha-D-glucopyranosylur
onic acid (PNP-GA) as substrates for the enzyme reaction. The crude en
zyme preparations originating from five basidiomycetes, such as Coriol
us pubescens, Irpex lacteus, Merulius tremellosus, and so forth, hydro
lyzed GA-2X, but they did not hydrolyze PNP-GA. The results lead to th
e conclusion that the two substrates are hydrolyzed by different enzym
es.