IDENTIFICATION OF NOVEL SINGLE-STRANDED-DNA BINDING-PROTEINS RECOGNIZING OCTAMER MOTIF

We have previously identified the region containing transcriptional en hancer in mouse immunoglobulin heavy chain (IgH) gene as an initiation region of DNA replication. The octamer sequence therein has been foun d to be essential for initiation of DNA replication as well as for tra nscription. Octamer-binding protein family, including Oct (OTF)-1, 2A, and 2B, bound to the element in double-stranded form. Here we identif ied in various cells novel proteins which bound to the upper strand of the octamer sequence in the IgH gene. Three proteins of 35, 60, and 1 25 KDa recognized the sequences. The proteins, which were not recogniz ed by an anti-Oct-1 antibody, may be involved in regulation of the ini tiation of DNA replication in the IgH gene. (C) 1994 Academic Press, I nc.