MOLECULAR-CLONING AND EXPRESSION OF THE GENE ENCODING HUMAN ANGIOTENSIN-II TYPE-2 RECEPTOR

The gene of human angiotensin II type 2 (AT(2)) receptor was isolated from a genomic DNA library prepared from human placenta. The coding re gion of the human AT(2) receptor gene was contained in a single exon c oding segment of the gene indicating an intronless structure of the co ding region. The amino acid sequence of human AT(2) receptor deduced f rom its nucleotide sequence has 363 amino acids and shows a high degre e of sequence identity to rat and mouse receptor sequences. Specific b inding of [I-125]Sar(1)Ile(8)-angiotensin II was demonstrated in COS-7 cells transfected with a plasmid containing the human AT(2) sequence. Scatchard analysis and ligand displacement profile were typical of th e AT(2) receptor. Reverse transcription-polymerase chain reaction anal ysis showed that AT(2) receptor mRNA was expressed in adult uterus and pheochromocytoma. (C) 1994 Academic Press, Inc.