Kd. Yang et Mf. Shaio, HYDROXYL RADICALS AS AN EARLY SIGNAL INVOLVED IN PHORBOL ESTER-INDUCED MONOCYTIC DIFFERENTIATION OF HL-60 CELLS, Biochemical and biophysical research communications, 200(3), 1994, pp. 1650-1657
A useful model for the study of monocytic differentiation has been the
induction of myeloid leukemic cell differentiation. Employing human H
L60 leukemic cells, we sought to clarify whether reactive oxygen inter
mediates (ROI) are involved in phorbol myristate acetate (PMA)-induced
monocytic differentiation. We found that hydroxyl radical (OH) scaven
ger pyrrolinodimethylthiocarbamate (PDTC), but not O-2(-), H2O2 or O-1
(2) sc avenger, suppressed PMA-mediated HL60 cell differentiation in a
dose dependent fashion (0.1 to 1.0 mu M). PDTC suppressed OH but not
O-2(-) or H2O2 production by HL60 cells stimulated with PMA (32 nM). A
ddition of PDTC before and right after, but not 16 hours after, PMA st
imulation inhibited PMA-induced HL60 cell differentiation. Taken toget
her, these findings indicate that OH, but not O-2(-), H2O2, or O-1(2),
is a critical early signal involved in the PMA-induced monocytic diff
erentiation of HL60 cells. (C) 1994 Academic Press, Inc.