PRODUCTION OF HUMAN PTH(1-34) VIA A RECOMBINANT-DNA TECHNIQUE

The production of hPTH(1-34) by site specific chemical cleavage of [Cy s(35)]hPTH(1-84) obtained by a biotechnology technique is described. T he amino-peptide bond of S-cyanylated cysteine residues in peptides or proteins is specifically cleaved by exposure to an alkaline solution (Stark, G. R. (1977) Methods Enzymol, 47, 129-132). However, when appl ying this method to [Cys(35)]hPTH(1-84), we observed many by-products. Formation of these by-products was suppressed using a short reaction in 0.03N NaOH containing 6M urea at low temperature, and hPTH(1-34) wa s specifically produced. The product was indistinguishable from standa rd hPTH(1-34) with respect to chemical and biological characterization . (C) 1994 Academic Press,