N. Cimolai et Dg. Mah, IMMUNODOMINANT ANTIGENS OF STREPTOCOCCUS-EQUISIMILIS SHARED BY OTHER BETA-HEMOLYTIC STREPTOCOCCI, Journal of Medical Microbiology, 40(5), 1994, pp. 323-329
Three immunodominant antigens of Streptococcus equisimilis (Lancefield
group C) with approximate mel, wts of 46, 66 and 105 kDa were recogni
sed by human serum IgG and IgA immunoblotting. These antigens were ide
ntified consistently by various human sera but immunoblots with IgA (h
eavy chain) and secretory IgA (J chain) from human respiratory secreti
ons gave more variable results. Antigens with similar migration rates
were demonstrated in S. pyogenes, large colony human biotype group G s
treptococci, and streptococci of groups C and G from the ''S. anginosu
s-milleri group''. Polyclonal antibody which was eluted from immunoblo
t substrates that contained the S. equisimilis 66-kDa antigen reacted
with the 60-kDa antigen of S. pyogenes. Both polyclonal and monoclonal
anti-vimentin antibodies identified the 46-kDa and 66-kDa antigens of
S. equisimilis. The homology of these antigens among beta-haemolytic
streptococci has the potential to complicate both a strategy for the u
tilisation of immunoblotting for diagnostic purposes and the understan
ding of how such antigens may be involved in the pathogenesis of post-
infectious sequelae.