SYNTHESIS OF A WATER-SOLUBLE SERINE-BASED NEOGLYCOLIPID WHICH CAN BE COVALENTLY-LINKED TO SOLID-PHASES

Authors
ELOFSSON M BRODDEFALK J EKBERG T KIHLBERG J
Citation
M. Elofsson et al., SYNTHESIS OF A WATER-SOLUBLE SERINE-BASED NEOGLYCOLIPID WHICH CAN BE COVALENTLY-LINKED TO SOLID-PHASES, Carbohydrate research, 258, 1994, pp. 123-133
Citations number
39
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
258
Year of publication
1994
Pages
123 - 133
Database
ISI
SICI code
0008-6215(1994)258:<123:SOAWSN>2.0.ZU;2-L
Abstract
N-alpha-Fmoc-serine pentafluorophenyl ester was glycosylated with perb enzoylated lactosyl bromide. The resulting product was coupled to a re sin functionalized with 6-aminohexanoic acid and then N-alpha-acylated to give a serine-based analogue of lactosylceramide. The water-solubl e neoglycolipid was covalently linked to microtiter plates via its car boxyl group and was recognized by a lactose-binding lectin in an ELISA .