ANALYSIS OF THE FRAGMENTS FROM COLLISION-INDUCED DISSOCIATION OF ELECTROSPRAY-PRODUCED PEPTIDE IONS USING A QUADRUPOLE ION-TRAP STORAGE REFLECTRON TIME-OF-FLIGHT MASS-SPECTROMETER
Bm. Chien et Dm. Lubman, ANALYSIS OF THE FRAGMENTS FROM COLLISION-INDUCED DISSOCIATION OF ELECTROSPRAY-PRODUCED PEPTIDE IONS USING A QUADRUPOLE ION-TRAP STORAGE REFLECTRON TIME-OF-FLIGHT MASS-SPECTROMETER, Analytical chemistry, 66(10), 1994, pp. 1630-1636
The capabilities of the ion trap storage/reflectron time-of-flight mas
s spectrometer (IT/reTOF) as a method for rapid structural analysis of
low levels of peptides with relatively high resolution are demonstrat
ed. The fragmentation generated by collision-induced dissociation (CID
) of etectrospray-produced ions of peptides between the capillary exit
and the skimmer of the electrospray source is analyzed by the IT/reTO
F. A resolution of 2000-3000 is obtained for the fragment ions dependi
ng upon the storage time and other conditions of the particular experi
ment. This resolution is sufficient to resolve the isotopic distributi
on of the fragment ions, including the multiply charged ions, thus pro
viding identification of the charge state. The resolution attained for
fragment ions is enhanced by the use of a buffer gas and storage capa
bilities of the trap. It is shown that for these CID spectra such reso
lution can be obtained on low picomole samples on this relatively simp
le, inexpensive instrument.