We examine the stability characteristics of a two-dimensional flow whi
ch consists initially of an inflexionally unstable shear layer on an f
-plane. Under the action of the primary instability, the vorticity in
the shear-layer initially coalesces into two Kelvin-Helmholtz vortices
which subsequently merge to form a single coherent vortex. At a seque
nce of times during this process, we test the stability of the two-dim
ensional flow to fully three-dimensional perturbations. A somewhat nov
el approach is developed which removes inconsistencies in the secondar
y stability analyses which might otherwise arise owing to the time-dep
endence of the two-dimensional flow. In the non-rotating case, and bef
ore the onset of pairing, we obtain a spectrum of unstable longitudina
l modes which is similar to that obtained previously by Pierrehumbert
& Widnall (1982) for the Stuart vortex, and by Klaassen & Peltier (198
5, 1989, 1991) for more realistic flows. In addition, we demonstrate t
he existence of a new sequence of three-dimensional subharmonic (and t
herefore helical) instabilities. After pairing is complete, the second
ary instability spectrum is essentially unaltered except for a doublin
g of length- and timescales that is consistent with the notion of spat
ial and temporal self-similarity. Once pairing begins, the spectrum qu
ickly becomes dominated by the unstable modes of the emerging subharmo
nic Kelvin-Helmholtz vortex, and is therefore similar to that which is
characteristic of the post-pairing regime. Also in the context of non
-rotating flow, we demonstrate that the direct transfer of energy into
the dissipative subrange via secondary instability is possible only i
f the background flow is stationary, since even slow time-dependence a
cts to decorrelate small-scale modes and thereby to impose a short-wav
e cutoff on the spectrum. The stability of the merged vortex state is
assessed for various values of the planetary vorticity f. Slow rotatio
n may either stabilize or destabilize the columnar vortices, depending
upon the sign off, while fast rotation of either sign tends to be sta
bilizing. When f has opposite sign to the relative vorticity of the tw
o-dimensional basic state, the flow becomes unstable to a new mode of
instability that has not been previously identified. Modes whose energ
y is concentrated in the vortex cores are shown to be associated, even
at non-zero f, with Pierrehumbert's (1986) elliptical instability. Th
rough detailed consideration of the vortex interaction mechanisms whic
h drive instability, we are able to provide physical explanations for
many aspects of the three-dimensionalization process.