CLONING AND SEQUENCE-ANALYSIS OF DNA GYRASE GENES FROM MYCOBACTERIUM-TUBERCULOSIS

Authors
MADHUSUDAN K RAMESH V NAGARAJA V
Citation
K. Madhusudan et al., CLONING AND SEQUENCE-ANALYSIS OF DNA GYRASE GENES FROM MYCOBACTERIUM-TUBERCULOSIS, Current Science, 66(9), 1994, pp. 664-667
Citations number
32
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Current Science → ACNP
ISSN journal
00113891
Volume
66
Issue
9
Year of publication
1994
Pages
664 - 667
Database
ISI
SICI code
0011-3891(1994)66:9<664:CASODG>2.0.ZU;2-#
Abstract
Using a heterologous DNA gyrase probe from Streptomyces sphaeroides, w e have cloned the gyrA and gyrB genes of Mycobacterium tuberculosis. T he M. tuberculosis gyrB gene has been sequenced and compared with the amino acid sequences of GyrB from other bacteria. There is a significa nt level (60-80%) of amino acid sequence homology between GyrB of M. t uberculosis and that of other bacterial species, indicating a high deg ree of conservation of this essential enzyme.