A. Sharma et Nsm. Quantrill, MEASUREMENT OF GLUCOSE USING FLUORESCENCE QUENCHING, Spectrochimica acta. Part A: Molecular spectroscopy, 50(6), 1994, pp. 1179-1193
A new spectroscopic procedure for the measurement of glucose concentra
tions is described which is based on substrate induced quenching (SIQ)
of an indicator florescence. The method exploits a novel photo reacti
on between thionine and NADH, the latter being generated due to the re
duction of NAD+ in an enzymic reaction between glucose dehydrogenase (
GDH) and glucose. The observed SIQ data was analysed using an empirica
l relation. A quenching constant of 1.8x10(3) (+/-100) M-1 is obtained
for the substrate induced quenching of thionine by glucose. The repor
ted method, which was investigated over the range 0-1000 muM, offers a
glucose detection limit of 2.2 muM. Various applications of the propo
sed scheme are discussed, including its use to construct a fibre optic
biosensor for glucose.